群馬大学 生体調節研究所 細胞構造分野
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准教授 原 太一 (2010年10月〜2017年3月)


研究課題

・遺伝子改変マウスを用いたエンドサイトーシスの生理的役割の解析

・細胞内脂質ホメオスタシスの分子機構の解析

・マウス初期胚発生における細胞内環境リモデリングの解析

・小胞体品質管理機構の解析

 

研究業績

原著論文


Autophagy-deficient mice develop multiple liver tumors.
Takamura A, Komatsu M, Hara T, Sakamoto A, Kishi C, Waguri S, Eishi Y, Hino O, Tanaka K, Mizushima N.
Genes Dev. 25(8):795-800 (2011)

Post-Translational Regulation and Nuclear Entry of TIMELESS and PERIOD Are Affected in New timeless Mutant.
Hara T, Koh K, Combs DJ, Sehgal A.
J Neurosci., 31(27):9982-90 (2011)

Ubiquitin accumulation in autophagy-deficient mice is dependent on the Nrf2-mediated stress response pathway: a potential role for protein aggregation in autophagic substrate selection.

Riley BE, Kaiser SE, Shaler TA, Ng AC, Hara T, Hipp MS, Lage K, Xavier RJ, Ryu KY, Taguchi K, Yamamoto M, Tanaka K, Mizushima N, Komatsu M, Kopito RR.

J Cell Biol. 1;191(3):537-52 (2010)

 

Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly.

Kaizuka T, Hara T, Oshiro N, Kikkawa U, Yonezawa K, Takehana K, Iemura S, Natsume T, Mizushima N.

J Biol Chem. 25;285(26):20109-16 (2010)

 

Atg101, a novel mammalian autophagy protein interacting with Atg13.

Hosokawa N, Sasaki T, Iemura S, Natsume T, Hara T, Mizushima N.

Autophagy. 5(7):973-9 (2009)

 

Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy.

Hosokawa N*, Hara T*, Kaizuka T, Kishi C, Takamura A, Miura Y, Iemura S, Natsume T, Takehana K, Yamada N, Guan JL, Oshiro N, Mizushima N.

Mol Biol Cell. 20(7):1981-91 (2009) * These authors contributed equally to this work.

 

Role of ULK-FIP200 complex in mammalian autophagy: FIP200, a counterpart of yeast Atg17?

Hara T, Mizushima N.

Autophagy. 1;5(1):85-7 (2009)

 

The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice.

Sou YS, Waguri S, Iwata J, Ueno T, Fujimura T, Hara T, Sawada N, Yamada A, Mizushima N, Uchiyama Y, Kominami E, Tanaka K, Komatsu M.

Mol Biol Cell. 19(11):4762-75 (2008)

 

FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells.

Hara T, Takamura A, Kishi C, Iemura S, Natsume T, Guan JL, Mizushima N.

J Cell Biol. 2008 May 5;181(3):497-510 (2008).

 

Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice.

Komatsu M, Waguri S, Koike M, Sou YS, Ueno T, Hara T, Mizushima N, Iwata J, Ezaki J, Murata S, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T, Uwayama J, Warabi E, Yoshida H, Ishii T, Kobayashi A, Yamamoto M, Yue Z, Uchiyama Y, Kominami E, Tanaka K.

Cell. 14;131(6):1149-63 (2007)

 

Intracellular quality control by autophagy: how does autophagy prevent neurodegeneration?

Mizushima N., Hara T.

Autophagy. 2(4), 302-4 (2006)

 

Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice.

Hara T., Nakamura K., Matsui M., Yamamoto A., Nakahara Y., Suzuki-Migishima R., Yokoyama M., Mishima K., Saito I., Okano H., Mizushima N.

Nature. 441(7095), 885-9 (2006)

 

Role of the UBL-UBA protein KPC2 in degradation of p27 at G1 phase of the cell cycle.

Hara T., Kamura T., Kotoshiba S., Takahashi H., Fujiwara K., Onoyama I., Shirakawa M., Mizushima N., Nakayama K.I.

Mol Cell Biol. 25 (21), 9292-303 (2005)

 

Molecular dissection of the interaction between p27 and Kip1 ubiquitylation-promoting complex, the ubiquitin ligase that regulates proteolysis of p27 in G1 phase.

Kotoshiba S., Kamura T., Hara T., Ishida N., Nakayama K.I.

J Biol Chem. 280 (18), 17694-700 (2005)

 

Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27Kip1 at G1 phase.

Kamura T., Hara T., Matsumoto M., Ishida N., Okumura F., Hatakeyama S., Yoshida M., Nakayama K., and Nakayama K.I.

Nature Cell Biol. 6 (12), 1229-35 (2004)

 

Kamura T., Hara T., Kotoshiba S., Yada M., Ishida N., Imaki H., Hatakeyama S., Nakayama K., Nakayama K.I.

Degradation of p57Kip2 Mediated by SCFSkp2-dependent Ubiquitylation

Proc Natl Acad Sci U S A. 100 (18), 10231-6 (2003)

 

Ishida N., Hara T., Kamura T., Yoshida M., Nakayama K., Nakayama K.I.

Phosphorylation of p27Kip1 on Serine 10 Is Required for Its Binding to CRM1 and Nuclear Export

J. Biol. Chem. 277 (17), 14355-8 (2002)

 

Hara T., Kamura T., Nakayama K., Oshikawa K., Hatakeyama S., Nakayama K.I.

Degradation of p27Kip1 at the G0-G1 Transition Mediated by a Skp2-independent Ubiquitination Pathway

J. Biol. Chem. 276 (52), 48937-3 (2001)

著書
原太一、水島昇. 栄養飢餓とオートファジー
細胞工学 (秀潤社) 28, p784-788 (2009)

原太一、水島昇. 神経変性疾患におけるオートファジーの役割
「神経変性疾患のサイエンス」高橋良輔編   南山堂 49-57 (2007)

原太一、水島昇. 哺乳類オートファジーの制御因子
蛋白質核酸酵素 (共立出版) 1484-1489 (2006)

原太一、水島昇. 神経細胞内浄化機構としてのオートファジー
細胞工学(秀潤社) 6号644-5 (2006)

原太一、水島昇. 細胞内品質管理機構
最新医学 (最新医学社) 9号1976-1981 (2004)

原太一、中山敬一. CDKインヒビターp27の分解制御機構
化学工業 (化学工業社) 7号19-25 (2003)

原太一、中山敬一. CDKインヒビターp27Kip1の分解制御と癌
Molecular Medicine 11号1288-96 (2002)